Insertion junction: LMJ.RY0402.122054_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122054
Chromosome:chromosome 7
Location:5180728
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g348450 (1 of 18) K13412 - calcium-dependent protein kinase (CPK) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAGTCGGTCCTGAAGCTGAACAGTCTG
Internal bar code:GCGAATGGAACCCCGCGTACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:723
LEAP-Seq percent confirming:99.7297
LEAP-Seq n confirming:369
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGCAATTCGGAACGTGT
Suggested primer 2:CCGAACAGTCGGAAGCTAAG