Insertion junction: LMJ.RY0402.122054_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.122054
Chromosome:chromosome 17
Location:1070563
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g703750 (1 of 1) PF14222 - Cell morphogenesis N-terminal (MOR2-PAG1_N) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGACTGCTGCCCAACGTGTCCACCACCTT
Internal bar code:ACACCGTCGCCACGGTGTAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:64.1814
LEAP-Seq n confirming:835
LEAP-Seq n nonconfirming:466
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGCAAAACACAGTTCGGT
Suggested primer 2:GTCACTGACCTGCTTCTGGG