Insertion junction: LMJ.RY0402.122054_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.122054
Chromosome:chromosome_17
Location:1070563
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre17.g703750 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CAGACTGCTGCCCAACGTGTCCACCACCTT
Internal bar code:ACACCGTCGCCACGGTGTAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:64.1814
LEAP-Seq n confirming:835
LEAP-Seq n nonconfirming:466
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGCAAAACACAGTTCGGT
Suggested primer 2:GTCACTGACCTGCTTCTGGG