Insertion junction: LMJ.RY0402.122064_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.122064
Chromosome:chromosome 7
Location:4908928
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g346317 GT90F39,GT90-39 GT90 family protein 39; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAGCAAGTCCTCGTTGTACGACTTGCC
Internal bar code:CGGGGTTGTCGACGCCACGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:99.3416
LEAP-Seq n confirming:1358
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGTTTGGAAAAGCGGTA
Suggested primer 2:GCCACACACATGCACACATA