| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122066 |
| Chromosome: | chromosome 9 |
| Location: | 4088237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393543 | HCP2 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCTAGACGCATAGACGAGATTAAGTCT |
| Internal bar code: | ACGTAGGGCACCGGACGCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 788 |
| LEAP-Seq percent confirming: | 99.3039 |
| LEAP-Seq n confirming: | 2853 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGGTTGCGGTACTCAT |
| Suggested primer 2: | TTGTTAAATGGTGTCCCCGT |