| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.122066 |
| Chromosome: | chromosome 9 |
| Location: | 4088240 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393543 | HCP2 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCACCCATAGAGGCGCACACACGCCTTG |
| Internal bar code: | GGGCTGCCAGGGATAAGGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 98.3607 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAGGTTGCGGTACTCAT |
| Suggested primer 2: | TTGTTAAATGGTGTCCCCGT |