Insertion junction: LMJ.RY0402.122087_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122087
Chromosome:chromosome 16
Location:3660253
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g688550 GSTS1,GST1 (1 of 3) 2.5.1.18//5.3.99.2 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase // Prostaglandin-D synthase / Prostaglandin-H(2) Delta-isomerase; Glutathione S-transferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATACATACCGTACAGTGCGTAAGCGTTAC
Internal bar code:AGCGAATTGGGTGCCCCTATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:268
LEAP-Seq percent confirming:98.9221
LEAP-Seq n confirming:1193
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCCTTTCTCCCTCCTCC
Suggested primer 2:TCGACTATCGTGTCTCACGC