| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122091 |
| Chromosome: | chromosome 6 |
| Location: | 4210767 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278236 | UMM12 | (1 of 1) PTHR10108:SF871 - METHYLTRANSFERASE; Putative ubiquinone / menaquinone biosynthesis methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAACTGCTCTGGCAATATGAAGACGGA |
| Internal bar code: | GGATGTGCGAAAGTTGCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 61.3518 |
| LEAP-Seq n confirming: | 708 |
| LEAP-Seq n nonconfirming: | 446 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATTGCTGCTTGTACTGC |
| Suggested primer 2: | TTTGTTTTGGATCGCATGAA |