| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122101 |
| Chromosome: | chromosome 11 |
| Location: | 3238725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479700 | DFO1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTCGGGGTGACACCGCAGGGTGATGG |
| Internal bar code: | GCGCAGGAATGGGGATGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 56.9874 |
| LEAP-Seq n confirming: | 681 |
| LEAP-Seq n nonconfirming: | 514 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCCATTCATGGAGTAGC |
| Suggested primer 2: | AAGAAGAAAGAAGGGAGGCG |