Insertion junction: LMJ.RY0402.122129_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122129
Chromosome:chromosome 1
Location:2589749
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g015100 CSB5 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACCTCTCACATGTACAAAACTTCCCAA
Internal bar code:GGGGGCATAAAAGATGCGGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:292
LEAP-Seq percent confirming:95.7908
LEAP-Seq n confirming:751
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACGACCTCAAACCAGACACC
Suggested primer 2:GACCATGCAGACCTGCTACA