Insertion junction: LMJ.RY0402.122156_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.122156
Chromosome:chromosome 9
Location:7144329
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g411975 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGCCCAGTGCTGCTGCGATGGTGGCGG
Internal bar code:AACTCTGCGGAATCCAGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTGACGTCATGCCTTTG
Suggested primer 2:TACGGCGTCTGCTGATACTG