| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122164 |
| Chromosome: | chromosome 8 |
| Location: | 1624862 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365400 | PRPL31 | Chloroplast ribosomal protein L31; (1 of 1) K02909 - large subunit ribosomal protein L31 (RP-L31, rpmE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTCCGCGCCTCTGCGTATCGACGTGCG |
| Internal bar code: | AACGGCGCCAGTGGTAGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 99.5885 |
| LEAP-Seq n confirming: | 968 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCTGTTCATGCTGTTC |
| Suggested primer 2: | GATGCTTGGAGGAATGGATG |