| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122175 |
| Chromosome: | chromosome 12 |
| Location: | 2573679 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505300 | (1 of 1) PF08317 - Spc7 kinetochore protein (Spc7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACAACATCGCTAGTCCTTTGTACACAC |
| Internal bar code: | GGCGAGTACAGACGAGACTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.5923 |
| LEAP-Seq n confirming: | 977 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGACTCGTTACCACATGC |
| Suggested primer 2: | ACGATTCAGAACCTGGATGC |