| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122195 |
| Chromosome: | chromosome_1 |
| Location: | 6680610 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g048000 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGAGCTAACCCTACCTCGTCTGGACTCGGG |
| Internal bar code: | CGGCCCCGCAAGTATTCGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 99.8902 |
| LEAP-Seq n confirming: | 910 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTGTCCACAGGGAATAC |
| Suggested primer 2: | AAAGGAGAAAGGCACAGCAA |