Insertion junction: LMJ.RY0402.122242_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122242
Chromosome:chromosome 6
Location:3771910
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278159 CRCO,CO,CON1,CONSTANS CONSTANS-like protein involved in circadian rhythms; (1 of 1) PF00643//PF06203 - B-box zinc finger (zf-B_box) // CCT motif (CCT) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTAATGATCTAGTTGGTCGAGTGACGAG
Internal bar code:ACGAGGAACGAGGACTCTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:963
LEAP-Seq percent confirming:99.0167
LEAP-Seq n confirming:6747
LEAP-Seq n nonconfirming:67
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCTGCTGGAAGTACTGGA
Suggested primer 2:AATCTCGCCAACTACCCCTT