| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122289 |
| Chromosome: | chromosome 9 |
| Location: | 6451524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAGTCGAGTGCGCTGAGTGGCAGTTGA |
| Internal bar code: | GTCACCTACTTTCCACTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 99.3003 |
| LEAP-Seq n confirming: | 6670 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCCCTAGACGAAAACCC |
| Suggested primer 2: | GCACATTGCGTTTCTTCCTT |