| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.122296 |
| Chromosome: | chromosome 16 |
| Location: | 5615107 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679750 | (1 of 2) PTHR17630//PTHR17630:SF43 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCTACCATAGCGGCTCGGGCGCCCTGGT |
| Internal bar code: | GTCAAAGGGACCCGTATCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 99.7373 |
| LEAP-Seq n confirming: | 2278 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACAGCAATAAAAGCCCC |
| Suggested primer 2: | ATTCAACCCGTGTAAGGCTG |