| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122306 |
| Chromosome: | chromosome 6 |
| Location: | 470003 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252700 | SPL2 | Splicing factor 3a, subunit 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAACGTGCAGGGCCGGCCTACGGGGGGG |
| Internal bar code: | GGGTGTAATCGGTACGCATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 899 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGGTGTGCTGTAGTGTA |
| Suggested primer 2: | GAGTGCGCAGTCTCCCTATC |