Insertion junction: LMJ.RY0402.122310_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.122310
Chromosome:chromosome 8
Location:4391858
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g381950 YAK1,TAR1 (1 of 1) K18670 - dual specificity protein kinase YAK1 [EC:2.7.12.1] (YAK1); DYRK-type protein kinase/Yet-another-kinase 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTGCTGACAAAGCCGGCGGTGGGCGTG
Internal bar code:TCAATCTACAACCCGAGGGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:91
LEAP-Seq percent confirming:98.6487
LEAP-Seq n confirming:146
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGACCAACACATCGTTC
Suggested primer 2:CATGATGATGGTGGAGGATG