Insertion junction: LMJ.RY0402.122323_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122323
Chromosome:chromosome 17
Location:2336755
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g714400 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGAGATGTGCTGGAACTGGAGCAGGAGC
Internal bar code:TGGTTGCTGGTTTTTGTCAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:920
LEAP-Seq percent confirming:99.6209
LEAP-Seq n confirming:1314
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCACTCGCCAAGATACC
Suggested primer 2:GTGCTCCTGCTCCTATCCAG