| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122415 |
| Chromosome: | chromosome 6 |
| Location: | 4864597 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278750 | UCP1 | (1 of 1) PTHR24089//PTHR24089:SF200 - FAMILY NOT NAMED // MITOCHONDRIAL UNCOUPLING PROTEIN 1; mitochondrial uncoupling protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGGCAGGATGCAGCTCAGTGGAGCGCT |
| Internal bar code: | AGAAGTGTTAAAGCATTTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 99.7699 |
| LEAP-Seq n confirming: | 5204 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGCCTATCGACCCTGGA |
| Suggested primer 2: | AGCTGAAGGGGTGGGTAGTT |