Insertion junction: LMJ.RY0402.122465_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122465
Chromosome:chromosome 7
Location:6001203
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g354600 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGGGTCCCCAACTTATCTACCTCGGAG
Internal bar code:GTAGTCCGTAAGGAGGCAGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:848
LEAP-Seq percent confirming:99.8318
LEAP-Seq n confirming:2967
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CAACATCCACCTGACGACAC
Suggested primer 2:AAAAGGTACCTCGTGGCCTT