| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122473 |
| Chromosome: | chromosome 12 |
| Location: | 5317010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529150 | PPP40,PP2C1,FAP314 | Flagellar Associated Protein 314; (1 of 1) PTHR13832:SF352 - PROTEIN PHOSPHATASE 2C 59-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTAGAGACAGGTTGCCGTTGATGGTCTG |
| Internal bar code: | AGCGGGCACAAGCCGAAAGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.8295 |
| LEAP-Seq n confirming: | 1757 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACCTGGGAGGGTTGTAA |
| Suggested primer 2: | CAGCATATGACACGGACTGG |