| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.122495 |
| Chromosome: | chromosome 12 |
| Location: | 3732089 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514850 | HSP90C | Heat shock protein 90C; (1 of 1) PTHR11528//PTHR11528:SF54 - HEAT SHOCK PROTEIN 90 // ENDOPLASMIN-LIKE PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATACGGTGCGGATTCAGCGCTGTAGCA |
| Internal bar code: | ATAATGGTTATCATATGTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 99.5663 |
| LEAP-Seq n confirming: | 2755 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTATGGCGAGAGCTTCTG |
| Suggested primer 2: | TGCATTCCACAACCACAACT |