| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122553 |
| Chromosome: | chromosome 7 |
| Location: | 5453473 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350550 | EEP6 | (1 of 3) PTHR22748//PTHR22748:SF4 - AP ENDONUCLEASE // DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE 2; putative exodeoxyribonuclease III | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACCACGGAAGCTCTTGTGGCGCAGTTC |
| Internal bar code: | ATACCGGTGCGGTGGCAGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.9177 |
| LEAP-Seq n confirming: | 2427 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGACTGCACGACCGAGT |
| Suggested primer 2: | TCCGTCACATGACCTTGAAA |