| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122561 |
| Chromosome: | chromosome 1 |
| Location: | 5509787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038900 | (1 of 5) PTHR11614:SF87 - MONOGLYCERIDE LIPASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGCCACGTTTGTTAGTGAAGTGGCTT |
| Internal bar code: | CGGACAGGGCGTGGCCGCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 99.7328 |
| LEAP-Seq n confirming: | 2986 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCATGTAAACAAAAGCGG |
| Suggested primer 2: | GCATCCGAAGGTTAAGGACA |