| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122565 |
| Chromosome: | chromosome 7 |
| Location: | 1786740 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325722 | (1 of 1) PTHR13215:SF0 - SUB1 HOMOLOG (S. CEREVISIAE); RNA polymerase coactivator | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGCCGTGCCTGCGTGTGCCCTGGCCT |
| Internal bar code: | CTGACAGTACCAATCATGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 99.8314 |
| LEAP-Seq n confirming: | 7699 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTATCGCTAACACGCCCAC |
| Suggested primer 2: | GTGTGTATGGGAGGTTTGGG |