| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.122583 |
| Chromosome: | chromosome 13 |
| Location: | 4211235 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592500 | (1 of 1) K00953 - FAD synthetase (FLAD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGATGATGCCGTTATCCACGGGGTACGA |
| Internal bar code: | ACGATCGCCTTGCAGGTTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 99.2347 |
| LEAP-Seq n confirming: | 389 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGTGCAATTTCTCTTG |
| Suggested primer 2: | TTGTTCAAGCTGCCAATCAG |