| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122585 |
| Chromosome: | chromosome 2 |
| Location: | 5631571 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109366 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCAACAGGCGCAGGCGCAGGCGCTGA |
| Internal bar code: | TTGGCGTCATGGATTCTCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 461 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACCGCATGAGAACTTCC |
| Suggested primer 2: | TTTGCAACTGCTACGGTGAG |