Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.122601 |
Chromosome: | chromosome_5 |
Location: | 3374563 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g240900 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTAAAAATGCACAGCCATTGTGCCCCACCC |
Internal bar code: | TTGCACAACTACGATTGGGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 645 |
LEAP-Seq percent confirming: | 99.7722 |
LEAP-Seq n confirming: | 876 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTAGCAAGCCATGCAACTGA |
Suggested primer 2: | CAGCAACAGCAGGCGTACTA |