| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122602 |
| Chromosome: | chromosome 12 |
| Location: | 1105416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490600 | (1 of 2) IPR009060//IPR015940 - UBA-like // Ubiquitin-associated domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAGTGCTCTTCTCCTACGCTTCTGGTT |
| Internal bar code: | CCGCCCTCCCATATCTATCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 93.617 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCCTCCTCCAAAGACAG |
| Suggested primer 2: | CATGCACACAAACAACTCCC |