| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122605 |
| Chromosome: | chromosome 16 |
| Location: | 1165674 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650500 | (1 of 1) K06195 - ApaG protein (apaG) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATTGGAGAGAGTTACGCCTTCTCCCCCC |
| Internal bar code: | CGGGCTGATGCTAGCGGAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 624 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGAAAGAAAGCATCCG |
| Suggested primer 2: | GCATGCGAAGTGAACAGAAA |