Insertion junction: LMJ.RY0402.122624_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.122624
Chromosome:chromosome 12
Location:3528392
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g513254 (1 of 2) K15340 - DNA cross-link repair 1A protein (DCLRE1A, SNM1A, PSO2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCCTTCTACTGGGAAGGATATCGCACAA
Internal bar code:GAATTTGAAGTGGCGTGAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:99.8804
LEAP-Seq n confirming:1670
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCCCAGGATGGACACTT
Suggested primer 2:CGACACACAACATCCTCCAC