Insertion junction: LMJ.RY0402.122624_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.122624
Chromosome:chromosome 12
Location:3528406
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g513254 (1 of 2) K15340 - DNA cross-link repair 1A protein (DCLRE1A, SNM1A, PSO2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAACCTGTCACGCACCAGGCGCTACACAA
Internal bar code:TATATGCCTTCATATCGCTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:775
LEAP-Seq percent confirming:99.6783
LEAP-Seq n confirming:4647
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCCCAGGATGGACACTT
Suggested primer 2:CGACACACAACATCCTCCAC