| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122645 |
| Chromosome: | chromosome 12 |
| Location: | 2123955 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509001 | RPK2 | RNA-polymerase-subunit kinase; (1 of 2) PTHR24055//PTHR24055:SF10 - MITOGEN-ACTIVATED PROTEIN KINASE // MITOGEN-ACTIVATED PROTEIN KINASE 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGACAGAAGTGTGGCGGCGGGGAAGCA |
| Internal bar code: | CGGGAGGTGGCCGGCAGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 99.7779 |
| LEAP-Seq n confirming: | 1797 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAGGAAAGTATGCGGCT |
| Suggested primer 2: | CGTCCTCGATTATCTCTCGC |