| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122661 |
| Chromosome: | chromosome 15 |
| Location: | 179918 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635100 | (1 of 2) IPR000104//IPR010402 - Antifreeze protein, type I // CCT domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTAACCGCGAAGCCAGGCGCCTCATCA |
| Internal bar code: | ATTCTTCGCGTCAGGGGGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.3729 |
| LEAP-Seq n confirming: | 2377 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACTATTCGTGTCCCCT |
| Suggested primer 2: | TAGGCAGCAACTGCAACAAC |