| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122735 |
| Chromosome: | chromosome 2 |
| Location: | 5854031 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGGGACGGAGAGAGCAACGCATTAGAC |
| Internal bar code: | TGAACTTGGCCCATGTTGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.6328 |
| LEAP-Seq n confirming: | 814 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAACTAATGTTGCTGCGG |
| Suggested primer 2: | TGCATATGGTCGTCGAATGT |