| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122744 |
| Chromosome: | chromosome 12 |
| Location: | 2779614 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g502950 | CPL15 | RNA-binding S4 domain-containing protein; (1 of 1) IPR002942//IPR017506 - RNA-binding S4 domain // Photosystem II S4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATACAACCGGTGTGATTTGGGCCCAA |
| Internal bar code: | ACGGATCCTGAGCGCCAATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 99.7147 |
| LEAP-Seq n confirming: | 3495 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTCACTGCGATCAGAC |
| Suggested primer 2: | TTCTTCGTCATCTCACACGC |