| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122772 |
| Chromosome: | chromosome 6 |
| Location: | 1962640 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263800 | SEE2 | Putative splicing endonuclease positive effector; (1 of 1) PTHR10887:SF369 - DNA2/NAM7 HELICASE FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATACTGTCTTTCCCCCTCACTGGCGGC |
| Internal bar code: | CCATCGAAAGAGTACTTCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 99.7385 |
| LEAP-Seq n confirming: | 6866 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACACCTGCTACAGGAA |
| Suggested primer 2: | CCTGTTGGCATGGGACTACT |