| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122797 |
| Chromosome: | chromosome 12 |
| Location: | 7868343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552850 | CGL77 | (1 of 2) 2.1.2.10 - Aminomethyltransferase / Tetrahydrofolate aminomethyltransferase; Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGGAGTGTGGATGGTTGCGCGGGCCT |
| Internal bar code: | CAATATCTCGTACCCAGGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 92.634 |
| LEAP-Seq n confirming: | 10614 |
| LEAP-Seq n nonconfirming: | 844 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTATGAGGGAGAGGGGC |
| Suggested primer 2: | GTCCCGTGATAACCCTCTCA |