| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122799 |
| Chromosome: | chromosome_2 |
| Location: | 7864380 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCCTGGAGCTTCACGGGTGGGGCGGCTG |
| Internal bar code: | GCGTCCACGAACGTAGCCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.9106 |
| LEAP-Seq n confirming: | 2235 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATTGAGGATGATGCGGAC |
| Suggested primer 2: | ATGGCCGTGTGTATCTCCTC |