Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.122825 |
Chromosome: | chromosome 7 |
Location: | 436842 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g315326 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACGGCCTGGCCGCAAAGGACGACCAA |
Internal bar code: | CACTTTTGAAGAGCATAAGTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 847 |
LEAP-Seq percent confirming: | 99.7597 |
LEAP-Seq n confirming: | 2906 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACATGGCAGCTGTAGCAAA |
Suggested primer 2: | TTCCCACCACCACTAACACC |