Insertion junction: LMJ.RY0402.122826_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122826
Chromosome:chromosome 2
Location:3400421
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095106 PGP1 (1 of 2) 2.7.8.5 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase / Phosphatidylglycerophosphate synthase; Phosphatidylglycerolphosphate synthase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGCCGAGACCAGCTGCGCGTCGTTGGCG
Internal bar code:GACCTACGGACGCCGCGAGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:663
LEAP-Seq percent confirming:99.8528
LEAP-Seq n confirming:1357
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGAACAGAAGCAAGCAC
Suggested primer 2:CCTCTCCTCTCACCACTTCG