| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122850 |
| Chromosome: | chromosome 11 |
| Location: | 2873239 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477800 | HDA10 | (1 of 1) PTHR10625//PTHR10625:SF119 - HISTONE DEACETYLASE // SUBFAMILY NOT NAMED; RPD3/HDA1 type histone deacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAACGTCTTTACACTCACCCGAGCACCC |
| Internal bar code: | GGATCCTTATGGACATCCTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.8392 |
| LEAP-Seq n confirming: | 1242 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAATTCAAGCGTCTTTG |
| Suggested primer 2: | CAATTTTATCGTGCGGTGTG |