Insertion junction: LMJ.RY0402.122850_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.122850
Chromosome:chromosome 16
Location:6036392
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g676350 (1 of 1) PTHR23073//PTHR23073:SF37 - 26S PROTEASE REGULATORY SUBUNIT // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATGGAAATGGTAGAGGAGGGTGTGGAGA
Internal bar code:GGAACGTGCTTGAGGAGACGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:348
LEAP-Seq percent confirming:98.1965
LEAP-Seq n confirming:7405
LEAP-Seq n nonconfirming:136
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCTTACTGGGAACGGTG
Suggested primer 2:CGTGTAAAGGCTGGCTTCTC