| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.122853 |
| Chromosome: | chromosome 1 |
| Location: | 1525557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007901 | (1 of 3) PTHR11206//PTHR11206:SF95 - MULTIDRUG RESISTANCE PROTEIN // MULTI ANTIMICROBIAL EXTRUSION FAMILY PROTEIN | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGCAGGTCAGGGCGCGGCGAGGGCGGG |
| Internal bar code: | AATTTGATTGGCTCCCGAGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.7606 |
| LEAP-Seq n confirming: | 3751 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTCCACCACTTTCCACT |
| Suggested primer 2: | GTTTCGCCTACTGTTTTGCC |