| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.122885 |
| Chromosome: | chromosome 8 |
| Location: | 4112929 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379700 | PHM1 | (1 of 1) 5.4.2.3 - Phosphoacetylglucosamine mutase / N-acetylglucosamine-phosphate mutase; Phosphoacetylglucosamine/phosphohexo mutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATGGTGCTTGGGGGTGTGCATGCGTG |
| Internal bar code: | CTGTTGAGGTTCCTCGGCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 96.9231 |
| LEAP-Seq n confirming: | 441 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCGCTTCATTATGGCGAC |
| Suggested primer 2: | GTTTGTATGTGCATGTCGCC |