| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.122888 |
| Chromosome: | chromosome 17 |
| Location: | 1651930 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708100 | (1 of 3) 2.4.2.8 - Hypoxanthine phosphoribosyltransferase / Transphosphoribosidase; Similar to Hypoxanthine-Guanine | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCACGAACTCGTTGGGGCACTGCGTGC |
| Internal bar code: | GTCAAGACATTCACTGTCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 281 |
| LEAP-Seq percent confirming: | 99.1525 |
| LEAP-Seq n confirming: | 234 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGCTATCCCGCTTCACT |
| Suggested primer 2: | GCCTCTGACAGCTCCGTATC |