Insertion junction: LMJ.RY0402.122896_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.122896
Chromosome:chromosome 3
Location:4206499
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g174050 MRP4 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCCCAACCCCAGCTGCCCCCCTCCCCT
Internal bar code:AAAAAAGGAAGGAAGGAGAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:81
LEAP-Seq percent confirming:30.303
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTTTTCGCACATGGAC
Suggested primer 2:GGAGGAGGACTAGGGGTGTC