| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.122931 |
| Chromosome: | chromosome_3 |
| Location: | 5006679 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g180450 | FAP215,FPN1 | 5\'-nucleotidase and Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCGCAGGCATCAAGGTCGGCATTGTGGG |
| Internal bar code: | TATATAGGGGTGGCCGGACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1181 |
| LEAP-Seq percent confirming: | 98.5207 |
| LEAP-Seq n confirming: | 666 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAATCTGCAGGACAAGC |
| Suggested primer 2: | GCTGATGTCCACCATCACAC |