Insertion junction: LMJ.RY0402.122931_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.122931
Chromosome:chromosome 3
Location:5006679
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g180450 FPN1,FAP215 (1 of 3) K01081 - 5'-nucleotidase (E3.1.3.5); Flagellar Associated Protein 215, 5'-nucleotidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCGCAGGCATCAAGGTCGGCATTGTGGG
Internal bar code:TATATAGGGGTGGCCGGACTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1181
LEAP-Seq percent confirming:98.5207
LEAP-Seq n confirming:666
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCACAATCTGCAGGACAAGC
Suggested primer 2:GCTGATGTCCACCATCACAC