| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.122932 |
| Chromosome: | chromosome 12 |
| Location: | 507641 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493200 | (1 of 39) IPR009003 - Peptidase S1, PA clan | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAGGCGCATGTGCCTCTCGTGCGGCTG |
| Internal bar code: | CCTCTGGCCGGGGTTGGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 93.1174 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCCACAGGGCAGAATA |
| Suggested primer 2: | AGCCGTCAGGTAGCAGGTAA |